師資
個人簡介：
龍艷萍，南方科技大學研究副教授。2007年本科畢業于西北農林科技大學，2015年博士畢業于中國科學院遺傳與發育生物學研究所，2016年加入南方科技大學生物系翟繼先課題組從事博士后研究并工作至今。2022年植物生物學女科學家學術交流會“優秀女青年獎”獲獎人?，F主要方向為基于高通量測序技術的植物單細胞測序和三代長測序技術的開發和應用以及基因表達調控機制相關研究。主導開發植物單細胞核全長測序技術FlsnRNA-seq、單分子水平全長新生RNA分析方法FLEP-seq以及用于擬南芥基因組多位點相互作用及關聯甲基化修飾的Pore-C 技術。目前已以第一作者（含共同）和共同通訊作者在Nature Plants、Nature protocols、PNAS、Genome Biology、Plant Biotechnology Journal和New Phytologist等國際主流學術期刊上發表多篇高水平研究論文。
研究領域：
1. 植物單細胞測序
2. 三代長測序技術的開發和應用
3. 基因表達調控研究
工作經歷：
2023至今 南方科技大學 研究副教授
2019 ~ 2023 南方科技大學 研究助理教授
2016 ~ 2019 南方科技大學 博士后
2015 ~ 2016 華大基因研究院 生物技術研究員
學習經歷：
2007 ~ 2015 發育生物學，博士，中國科學院遺傳與發育生物學研究所
2003 ~ 2007 生物技術，本科，西北農林科技大學
所獲榮譽：
2022 中國植物生理與植物分子生物學學會“優秀女青年”
代表文章：
(*co-first authors, #corresponding author)
1. Zhang K.*, Zhao X.*, Zhao Y., Zhang z., Liu Z., Liu Z., Yu Y., Li Z., Ma Y., Dong Y., Pang X., Jin X., Li N., Liu B., Wendel J., Zhai J., Long Y.#, Wang T.#, Gong L.#. (2023) Cell type-specific cytonuclear coevolution in three allopolyploid plant species. PNAS. 120(40):e2310881120.
2. Mo W.*, Shu Y.*, Liu B. Long Y., Li T., Cao X., Deng X. #, Zhai J.# (2023) Single-molecule targeted accessibility and methylation sequencing of centromeres, telomeres and rDNAs in Arabidopsis. Nature Plants. 9, 1439–1450.
3. Liu Z.*, Yang J.*, Long Y.*, Zhang C.*, Wang D., Zhang X., Dong W., Zhao L., Liu C., Zhai J.#, and Wang E.# (2023) Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago. Nature Plants. https://doi.org/10.1038/s41477-023-01524-8
4. Liu Z.*, Kong X.*, Long Y.*, Liu S., Zhang H., Jia J., Cui W., Zhang Z., Song X., Qiu L., Zhai J.#, Yan Z.# (2023) Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule maturation. Nature Plants. https://doi.org/10.1038/s41477-023-01387-z.
5. Qin Y., Long Y., Zhai J.# (2022) Genome-wide characterization of nascent RNA processing in plants. Current Opinion in Plant Biology. 69:102294.
6. Jia J.*, Lu W.*, Liu B., Yu Y., Jin X., Shu Y., Long Y., Zhai J.# (2022) An atlas of plant full-length RNA reveals tissue-specific and evolutionarily-conserved regulation of poly(A) tail length. Nature Plants. 8(9):1118-1126.
7. Li Z.*, Long Y.*, Yu Y., Zhang F., Zhang H., Liu Z., Jia J., Mo W., Tian S. Z., Zheng M., Zhai J.# (2022) Pore-C simultaneously captures genome-wide multi-way chromatin interaction and associated DNA methylation status in Arabidopsis. Plant Biotechnology Journal. 20(6):1009-1011.
8. Yu Y.*, Zhang H.*, Long Y., Shu Y., Zhai J.# (2022) Plant Public RNA-seq Database: a comprehensive online database for expression analysis of ~45?000 plant public RNA-Seq libraries. Plant Biotechnology Journal. 20, 806-808.
9. Long Y.*, Jia J.*, Mo W., Jin X., Zhai J.# (2021) FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing. Nature Protocols. 16, 4355-4381.
10. Long Y.*, Liu Z.*, Jia J.*, Mo W., Fang L., Lu D., Liu B., Zhang H., Chen W., Zhai J.# (2021) FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants. Genome Biology. 22, 66.
11. Mo W.*, Liu B.*, Zhang H., Jin X., Lu D., Yu Y., Liu Y., Jia J., Long Y., Deng X., Cao X., Guo H., Zhai J.# (2021) Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing. Genome Biology. 22(1):322.
12. Hu D.*, Yu Y.*, Wang C.*, Long Y., Liu Y., Feng L., Lu D., Liu B., Jia J., Xia R., Du J., Zhong X., Gong L., Wang K.#, Zhai J.# (2021) Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions. The Plant Cell. 33, 2950-2964.
13. Jia J.*, Ji R.*, Li Z., Yu Y., Nakano M., Long Y., Feng L., Qin C., Lu D., Zhan J., Xia R., Meyers BC, Liu B.#, Zhai J.# (2020) Soybean DICER-LIKE2 regulates seed coat color via production of primary 22-nucleotide small interfering RNAs from long inverted repeats. The Plant Cell. 32, 3662-3673.
14. Jia J.*, Long Y.*, Zhang H., Li Z., Liu Z., Zhao Y., Lu D., Jin X., Deng X., Xia R., Cao X., Zhai J.# (2020) Post-transcriptional splicing of nascent RNA contributes to widespread intron retention in plants. Nature Plants 6, 780-788.
15. Long Y.-P.*, Xie D.-J.*, Zhao Y.-Y., Shi D.-Q.#, and Yang W.-C.# (2019) BICELLULAR POLLEN 1 is a modulator of DNA replication and pollen development in Arabidopsis. New Phytologist. doi: 10.1111/nph.15610.
16. Zhang Y.*, Harris C.J.*, Liu Q.*, Liu W., Ausin I., Long Y., Xiao L., Feng L., Chen X., Xie Y., Chen X., Zhan L., Feng S., Li J., Wang H.#, Zhai J.# (2018) Large-scale comparative epigenomics reveals hierarchical regulation of non-CG methylation in Arabidopsis. PNAS. 115, E1069.
17. Fang H., Liu Z., Long Y., Liang Y., Jin Z., Zhang L., Liu D., Li H., Zhai J.# and Pei Y.# (2017) The Ca2+/calmodulin2- binding transcription factor TGA3 elevates LCD expression and H2S production to bolster Cr6+ tolerance in Arabidopsis. The Plant Journal. 91, 1038-1050.
18. Kuo H.Y., Jacobsen E.L., Long Y., Chen X., and Zhai J.# (2017) Characteristics and processing of Pol IV-dependent transcripts in Arabidopsis. Journal of Genetics and Genomics. 44, 3-6.
19. Wang, S.-Q., Shi, D.-Q., Long, Y.-P., Liu, J., and Yang, W.-C.# (2012) GAMETOPHYTE DEFECTIVE 1, a putative subunit of RNases P/MRP, is essential for female gametogenesis and male competence in Arabidopsis. PloS one. 7, e33595.
專利：
1. 新型冠狀病毒樣顆粒及其制備方法和應用，翟繼先，龍艷萍，賈津布，溫艦宇，鹿東東，申請號：202110333400.4，發明專利
2. 一種帶編碼的凝膠微粒及其制備方法和應用，翟繼先，肖麗丹，莫偉鵬，龍艷萍，申請號：202010047034.1，發明專利
3. 單細胞基因組測序用的DNA文庫的構建方法，翟繼先，龍艷萍，肖麗丹，張飛，鹿東東，專利號：ZL 2019 1 1311804.2，授權日：2023-05-05發明專利